In preparing a Fifth Edition, we have considerably extended the coverage of two dimensional NMR and introduced for the first time in the book the perwerful techniques of TOCSY and long-range 'H "C COSY (Chapter 1 In the chapter on mas spectrometry (Chapter 4), we have introduced the new methods of elec trospray sonization (ESI) and matrix-assisted laser desorption (MALDI and illustrated how these methods, when coupled with ion analys in quadrupele Fourier transform ion-cyclotron resonance (FT ICK), or time-of-fight (TOP) instruments can be used to determine the molecular weights of very large and highly polar molecules. Beshinchi nashrni tayyorlashda biz ikki o'lchovli NMR qamrovini sezilarli darajada kengaytirdik va kitobda birinchi marta TOCSY va uzoq masofali 'H "C COZY" ning ajoyib usullarini kiritdik (1-bob Mas-spektrometriya bo'limida (bo'lim). 4), biz elektr trospray sonizatsiyasi (ESI) va matritsa yordamida lazerli desorbsiyaning (MALDI) yangi usullarini joriy qildik va bu usullar to'rtburchak Furyeda ion tahlillari bilan birgalikda ion-siklotron rezonansini (FT ICK) yoki vaqtni qanday o'zgartirishini tasvirlab berdik. -of-fight (TOP) asboblari juda katta va yuqori qutbli molekulalarning molekulyar og'irliklarini aniqlash uchun ishlatilishi mumkin.